Research
Research interests
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Physiology and patho-physiology of electrolyte transport in kidney, respiratory tract, and intestine.
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Patho-physiology and molecular biology of? polycystic kidney disease.
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?Patho-physiology and molecular biology of cystic fibrosis and other inflammatory airway diseases.
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Development of novel ways for the treatment of asthma and cystic fibrosis lung disease. Exploring the possibility of targeting alternative ion channels.
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Role of ion channels for cell proliferation and cancer.
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?Role of ion channels in inflammatory diseases.
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?Contribution of TMEM16 proteins to regulated cell death, e.g. ferroptosis, apoptosis, and pyroptosis.
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Methods used
????? Electrophysiology
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-????????? Open and short circuit measurements in Ussing chambers
-????????? Patch clamp techniques in all applications
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????? Fluorescence based techniques
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-????????? Immunostaining of cellular components, soluble and membrane proteins in ????? Epithelial and non-epithelial cells
-????????? Fluorescence ratio measurements of intracellular pH, Ca2+ and Cl-
-????????? Confocal microscopy and video imaging
-????????? Iodide quenching of YFP fluorescence in plate reader and single cell microscopy
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????? Cell biological techniques
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-????????? Primary and permanent cell culture of epithelial and non-epithelial cells
-????????? S2 techniques including viral transfection, infectious agents
-????????? Cell tracking by HoloMonitor M4 Microscopy
-????????? Casy flow cytometry
-????????? Accury flow cytometry 4 wavelengths
-????????? Reconstitution of membrane proteins in liposomes
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????? Protein biochemistry & Molecular biology
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-????????? Western blotting
-????????? Immunoprecipitation, coimmunoprecipitation
-????????? DNA-cloning techniques
-????????? site directed mutagenesis
-????????? PCR - applications
-????????? DNA sequencing
-????????? siRNA techniques
-????????? CRISPR-Cas technology
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????? Knockout technology
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-????????? In vivo and ex vivo animals studies
-????????? Conventional and Cre/loxP knockout mice
-????????? Inducible Cre/loxP knockout mice
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AG Kunzelmann - 04.05.2021 09:31 
