topic
Ribosomes, the cellular factories for protein production, are among the most intricate and complex ribonucleoprotein (RNP)-assemblies in the cell. Their biogenesis is tightly regulated and requires the coordinated activity of many cellular processes and can therefore be considered as a well-suited model system to study mechanisms of biogenesis of RNP complexes. Synthesis of the majority of ribosomal RNAs in eukaryotes is executed by a highly specialized RNA polymerase, Pol I. Our major interests are to understand mechanisms and regulation of the RNA polymerase I transcription machinery and to study how rRNA synthesis is coupled to rRNA maturation and assembly of pre-ribosomes.
Our goals are to reconstitute transcription initiation, elongation and termination from a minimal transcription system, to understand the roles of single subunits of the transcription machinery and to compare features of Pol I transcription to other nuclear RNA polymerases.
Team
 | Joachim Griesenbeck, Principal investigatoremail:? joachim.griesenbeck (at) ur.de |
 | Philipp Milkereit, Principal investigatoremail:? philipp.milkereit (at) ur.de |
 | Jonas Pr?ll, PhD studentemail:? jonas.proell (at) ur.de |
 | Herbert Tschochner, Principal investigatoremail:? herbert.tschochner (at) ur.de |
Publications
Funding
?
-
since July 2011
DFG collaborative research Center SFB960 at the University of Regensburg: Ribosome formation: principles of RNP biogenesis and control of their function
Project A1: Analyses of the RNA polymerase I machinery: specific transcriptional mechanisms and comparison with other RNA polymerases
collaboration
Christophe Carles, CEA Saclay, Gif-sur-Yvette, France.
Olivier Gadal, Laboratoire de Biologie Moléculaire Eucaryote, Université Paul Sabatier, Toulouse III, France.
Thomas Moss, Centre de Recherche en Cancérologie, Université Laval, Quebec, Canada.
Patrick Schultz, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France.
